Transient absorption allows us to follow the evolution of molecular, aggregate or material systems on the ground and excited states as a function of femtosecond to nanosecond time delays. The principle of ultrafast transient absorption spectroscopy. In transient absorption spectroscopy, a fraction of the molecules is promoted to an electronically excited state by means of an excitation (or pump) pulse. These methods are collectively referred to as pump-and-probe methods although, depending on the time …

Author information: (1)Department of Physics and Astronomy, Faculty of Sciences, VU University Amsterdam, De Boelelaan 1081, Amsterdam, The Netherlands. Transient absorption techniques rely on the use of a probe beam that interrogates the sample before and after excitation with a pulsed laser (pump beam), and monitors absorbance changes either at a selected wavelength (kinetics) or simultaneously at several wavelengths (transient spectra).

Ultrafast transient absorption spectroscopy The principle of ultrafast transient absorption spectroscopy The process of energy transfer in a photosynthetic mem-brane typically takes place on a time scale from less than 100 fs to hundreds of ps (Sundstro¨m et al. Ultrafast transient absorption spectroscopy: principles and application to photosynthetic systems. The implementation of transient absorption mode in pump—probe microscopy will allow ground-state kinetics to be directly monitored. The process of energy transfer in a photosynthetic membrane typically takes place on a time scale from less than 100 fs to hundreds of ps (Sundström et al. 1994).The advent of ultrashort tunable laser systems in the early 1990s has opened up a new and … 1994). 1999; Van Amerongen and Van Grondelle 2001; Van Grondelle et al. 1999; Van Amerongen and Van Grondelle 2001; Van Grondelle et al. The advent of ultrashort tunable laser systems in the Depending on the type of experiment, this fraction typically ranges from 0.1% to tens of percents. Berera R(1), van Grondelle R, Kennis JT. A weak probe pulse (i.e., a pulse that has such a low intensity that multiphoton/multistep processes are avoided during probing) is sent through the sample …

Transient absorption spectroscopy, a special type of pump-probe spectroscopy, is a fundamental example of nonlinear spectroscopy.

The addition of two-photon excitation would improve the microscope spatial resolution as well as making wavelength-dependent spectroscopy possible.

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