Hydrophobic interaction chromatography (HIC) is an alternative to reversed phase chromatography for exploiting the hydrophobic properties of proteins. Hydrophobic Interaction and Reversed Phase Chromatography – Principles and Methods Hydrophobic Interaction and Reversed Phase Chromatography Principles and Methods … It was first described by Shepard and Tiselius in 1949 using the term “salting out chromatography.” Chromatography is a laboratory technique for the separation of a mixture.

Hydrophobic Interaction and Reversed Phase Chromatography – Principles and Methods Hydrophobic Interaction and Reversed Phase Chromatography Principles and Methods GE Healthcare

Hydrophilic interaction chromatography (or hydrophilic interaction liquid chromatography, HILIC) is a variant of normal phase liquid chromatography that partly overlaps with other chromatographic applications such as ion chromatography and reversed phase liquid chromatography.HILIC uses hydrophilic stationary phases with reversed-phase type eluents.

5 th Brooks Cole; 1997. Hydrophobic Interaction and Reversed Phase Chromatography Principles and Methods 11-0012-69 2-D Electrophoresis using immobilized pH gradients Principles and Methods 80-6429-60 Microcarrier Cell Culture Principles and Methods 18-1140-62 GST Gene Fusion System Handbook 18-1157-58 Affinity Chromatography Principles and Methods 18-1022-29
Please add to the list by commenting below! Hydrophobic Interaction and Reversed Phase Chromatography: Principles and Methods. Affinity Chromatography Principles and Methods 18-1022-29 Hydrophobic Interaction Chromatography Principles and Methods 18-1020-90 Gel Filtration Principles and Methods 18-1022-18 Handbooks from Amersham Pharmacia Biotech Reversed Phase Chromatography Principles and Methods 18-1134-16 Expanded Bed Adsorption Principles and Methods 18-1124-26 … The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase. Hydrophobic interaction chromatography (HIC) is a widely used chromatographic technique that finds application at different stages of downstream processing. TsKgel is available as bulk BibTeX @MISC{Healthcare_hydrophobicinteraction, author = {Ge Healthcare and Ge Healthcare and Europe Gmbh and Munzinger Strasse and Ge Healthcare and Amersham Place and Little Chalfont and Buckinghamshire Hp Na and Ge Healthcare and Bio-sciences Kk and Ge Healthcare and Bio-sciences Ab}, title = {Hydrophobic Interaction and Reversed Phase Chromatography Principles and Methods}, year = … The general principles of the most commonly used modes are outlined here. Genentech, Inc. Introduction. Further Reading.


Interaction principle According to Hydrophobic Interaction and Reversed Phase Chromatography Principles and Methods - GE Healthcare 4 Close to the surface of the hydrophobic ligand and solute, the water molecules are more highly ordered than in the bulk water and appear to “shield off” the hydrophobic ligand and solute molecules. Skoog DA, Holler FJ, Nieman, TA. Tosoh Bioscience offers a comprehensive line of media and pre­ packed columns for all common modes of liquid chromatography including ion­exchange, hydrophobic and hydrophilic interaction, reversed phase, size exclusion and affinity. Hydrophobic interaction chromatography (HIC) is an analytical technique that utilizes the hydrophobic properties of molecules to achieve their separation 1.In this type of chromatography, sample molecules with hydrophobic portion(s) interact with and bind to the hydrophobic groups (e.g., phenyl groups) attached to the column stationary phase.


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