Size exclusion chromatography (SEC) or gel permeation chromatography separates clusters according to their hydrodynamic volume (diameter). Size Size exclusion chromatography (SEC), also called gel filtration (GF) Hydrophobicity Hydrophobic interaction chromatography (HIC) Reversed phase chromatography (RPC) Charge Ion exchange chromatography (IEX) Biorecognition (ligand specificity) Affinity chromatography (AC) Isoelectric point (pI) Chromatofocusing (CF) Fig I.1. Size-exclusion chromatography (SEC) is a mode of chromatography that separates molecules according to their hydrodynamic radius. Please change the country on your profile in order to switch to another country store. What is size exclusion chromatography? 31 In SEC, smaller clusters diffuse further into porous microgels in the column and as a result spend a longer time in the column than larger clusters; that is, larger clusters elute faster than smaller clusters. The elution order is inversely proportional to the proteins hydrodynamic radius (the biggest molecules are excluded first). The stationary phase consists of spherical, porous particles with a carefully controlled pore size through which molecules diffuse, based on their size difference, using an aqueous buffer as the mobile phase. 31 In SEC, smaller clusters diffuse further into porous microgels in the column and as a result spend a longer time in the column than larger clusters; that is, larger clusters elute faster than smaller clusters. Other articles where Size-exclusion chromatography is discussed: separation and purification: Exclusion and clathration: Size-exclusion chromatography (SEC) has proved effective for the separation and analysis of mixtures of polymers. Size-exclusion chromatography (SEC) is a mode of chromatography that separates molecules according to their hydrodynamic radius. In this method the largest molecules emerge from the chromatographic column first, because they are unable to penetrate the porous matrix of the support. Dialysis, anion exchange, and size exclusion chromatography were used to produce a fraction consisting predominantly of saponin, also known as QuilA (Dalsgaard, 1974). Other articles where Size-exclusion chromatography is discussed: separation and purification: Exclusion and clathration: Size-exclusion chromatography (SEC) has proved effective for the separation and analysis of mixtures of polymers.

The aqueous separation techniques used were useful to purify the total saponin fraction, but failed to separate individual saponins due to the formation of mixed micelles. Cela nécessitera quelques explications, mais ça commence par une histoire un peu comme ça : D'abord vous solvatez le polymère, généralement dans un solvant appelé tetrafluoroéthylène, ou THF pour faire plus court. SEC – Size Exclusion Chromatography. With this technic molecules are discriminated according to their size. The gel consists of spherical beads containing pores of a specific size distribution. In this method the largest molecules emerge from the chromatographic column first, because they are unable to penetrate the porous matrix of the support.

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